Available ZH-attP lines and their genomic location of the attP landing site

For an exact analysis of the location of an attP landing site, or the distances to neighboring genes and the like, we provide here the flanking sequences of 25 attP lines. The given genomic sequences (5'-3' direction) immediately flank the landing site at the mariner 5' terminal repeat (m5'), which lies at the 3' end of the landing site construct (indicated in red, Figure below).

ZH-attP line chrom. 1
zh-1E X 1E
zh-2A X 2A sequence
zh-3Aa X 3A
zh-3Ab X 3A
zh-3B X 3B sequence
zh-5D X 5D
zh-6E X 6E sequence
zh-14F X 14F
zh-20C X 20C sequence
zh-X X-linked
ZH-attP line chrom. 2
zh-21F 2L 21F
zh-22A 2L 22A sequence
zh-27F 2L 27F
zh-30A 2L 30A sequence
zh-30B 2L 30B
zh-32C 2L 32C
zh-35B 2L 35B sequence
zh-36B 2L 36B sequence
zh-38D 2L 38D
zh-44F 2R 44F
zh-45A 2R 45A
zh-46A 2R 46A
zh-46D 2R 46D
zh-49B 2R 49B
zh-49D 2R 49D
zh-50C 2R 50C
zh-51C 2R 51C sequence
zh-51D 2R 51D sequence
zh-52E 2R 52E
zh-58A 2R 58A sequence
zh-59D 2R 59D
zh-60E 2R 60E
ZH-attP line chrom. 3
zh-62B 3L 62B sequence
zh-64A 3L 64A sequence
zh-67C 3L 67C
zh-68E 3L 68E sequence
zh-70C 3L 70C
zh-75C 3L 75C sequence
zh-75D 3L 75D
zh-82F 3R 82F
zh-83A 3R 83A
zh-83D 3R 83D sequence
zh-85D 3R 85D
zh-85Ea 3R 85E
zh-85Eb 3R 85E
zh-85F 3R 85F
zh-86Da 3R 86D sequence
zh-86Db 3R 86D
zh-86Dc 3R 86D sequence
zh-86Fa 3R 86F sequence
zh-86Fb 3R 86F sequence
zh-87E 3R 87E
zh-88A 3R 88A sequence
zh-88D 3R 88D
zh-89E 3R 89E
zh-91B 3R 91B
zh-92Aa 3R 92A sequence
zh-92Ab 3R 92A
zh-93D 3R 93D
zh-94D 3R 94D sequence
zh-96E 3R 96E sequence
ZH-attP line chrom. 4
zh-102D 4 -102D sequence

The names of the ZH-attP lines indicate the cytological position of the attP landing site. In cases where we have more than one line for the same cytological region we distinguish the lines by adding an "a" or "b" etc. at the end. ZH-attP-X could be mapped to the X-chromosome, but due to three X-chromosomal hits not to a specific cytological region yet. The lines provided with a sequence contain a putative intergenically located landing site (according annotated genes or GCs provided in Ensembl v41). Recently, Venken et al. (2006) also generated a considerable number of attP sites, which are marked by yellow, with a P-element strategy.

Targeting frequencies of various attP sites

The targeting frequencies found in the following table are based on doubly heterozygous flies, with one exception (you will find the details to the injection setup below the table).

ZH-attP line integration frequency (%) Eye color
ZH-attP-2A 0 Orange
ZH-attP-22A 39 Light orange
ZH-attP-30A† 41 Orange
ZH-attP-35B 40 Light orange
ZH-attP-36B 57 Orange
ZH-attP-51C 42 Light orange
ZH-attP-51D 60 Red
ZH-attP-58A 49 Light orange
ZH-attP-62B 39 Light orange
ZH-attP-64A 54 Orange
ZH-attP-68E 37 Light orange
ZH-attP-75C 37 Light orange
ZH-attP-86Fa 28 Red
ZH-attP-86Fb 43 Orange
ZH-attP-96E 44 Orange
ZH-attP-102D 45 Red

Females homozygous for the vas-phiC31 construct, located at 102D on the fourth chromosome, were crossed to males homozygous (or hemizygous) for an indicated attP site, and the offspring were injected with pUAS-lacZattB (215 ng/µl). Since line ZH-attP-2A has the attP site on the X chromosome, G0 males of this line are devoid of an attP site and therefore are not expected to give rise to transgenic offspring (G0 females of this line gave transgenic offspring).
Eye colors were determined three days after eclosion (heterozgous state). All flies transgenic for the injected plasmid reveal a reddish punctate color at the inner side of each of the three ocelli; this additional phenotype served as a convenient confirmation for transgenic flies in the cases of light orange eye color.  "Light orange" is in some cases hard to detect and it is helpful to screen for transformants of such attP lines only a few days after eclosion.
Landing site ZH-attP-30A, though homozygous viable on its own, turned out to be homozygous lethal upon integration of UAS-lacZattB.